It took me forever to figure out how to use this program. Not a good tool for a novice looking for a 'quick analysis'. There are lots of options that can be changed: axes, titles, etc, to customize the graphs, but these features take a very long time to find and result in a time consuming endeavor in the beginning. It is really handy how the stats are done all in one graph. Unlike Excel, you don't need to create multiple sheets of info in order to get the analysis you want, one set of data can be manipulated to do everything. I love that it easily does stats for you right in your graphed data. There are a ton of features to this program. My computer has 8GB RAM and good processor and still takes a lot to generate charts and analysis. Also, dose-response curves deserve more information and details about real life examples as well.Ībout the software specifically, I would suggest to make it lighter, or develop a light version. However, IC50 calculation tutorials could be improved adding real life examples with common absorbance or fluorescence experiments. GraphPad tutorial is good and very informative. Figures are amazing and you can easily add standard deviation, which is a must to in scientific publications. I've tried other software before and GraphPad is way easier to use and complete. It's pretty easy to import data and replicates and perform analysis over these replicates.

That's an incredible advantage for people who's learning and selecting their 'go-to' statistic software. GraphPad allows you to have one month free trial with all the features. It's an amazing software and it allowed me to transition from Excel and Origin and only use GraphPad now. I've also generated my charts, figures and reports directly to publish in peer-reviewed journals because of their quality. Comments: I have used GraphPad to calculate IC50, EC50 in fluorescence-based assays for my scientific project.

How to use graphpad prism